Directed protein evolution-a method that was awarded the Nobel Prize in chemistry in 2018 and it is often used in protein engineering to improve the properties of proteins.

Mutant library is a useful tool for protein/antibody engineering applications. Mutant libraries consist of large numbers of individual-sequences clones which can improve effort during the screening process and precisely target the interested genes. In this method, several parental DNA sequences are being recombined, resulting in protein variants. GenCefe offers site-directed mutagenesis libraries, scanning point mutation libraries, and combinatorial mutagenesis libraries for your downstream protein/antibody engineering applications.

Advantages of Mutant Library

• Various mutation library types: site-directed mutagenesis libraries, scanning point mutation libraries, and randomized library
• Expertise in gene synthesis and guarantee 100% sequence accuracy
• Customized mutation library: Clone the gene into the desired vector

The Types of Mutant Libraries

1.Site-Directed mutagenesis libraries

Any given residue can be substituted with any of the other 19 common amino acids, creating systematic combinations of amino acid mutations that reveal any significant pattern.

2.Scanning Point Mutant libraries

By replacing each amino acid with all 20 amino acids simultaneously to improve protein function.

3.Combinatorial mutagenesis library

Introducing random substitutions of the full-length gene in a synthetic DNA fragment.

Deliverables of Mutation Libraries

• Up to 10 µg of mutation constructs
• Mixed-mutagenesis library up to 20 variants for each position, delivered as one small library
• Separated variants (optional)
• Sequence verification information
• Statistical analysis of nucleotide distribution at the mutation site